Synthesis, characterization, and antioxidant activity in vitro of selenium-Euryale ferox Salisb. polysaccharide

Abstract In order to obtain and explore selenide composed of selenium polysaccharide, three parameters were selected optimize the synthesis process selenium- Euryale ferox Salisb. polysaccharide (Se-ESPS) by Box-Behnken design. Furthermore, ESPS-B1 separated from ESPS was selenitized Se-ESPS-B1 optimal process, then characterization, antioxidant activity in vitro explored. The result manifested that Se content Se-ESPS (2.915 ± 0.03) mg/g according (reaction time at 5 h, reaction temperature 81 ℃, weight ratio Na 2 SeO 3 0.9 g/g). A series detection results indicated characterizations apparently distinguished ESPS-B1. Moreover, experiments demonstrated could exert scavenging DPPH, ABTS,·OH, increasing reduction ability. conclusion, is an effective approach harvest selenium-polysaccharide, may be utilized as a potential or nutritional supplement.